Since ox-LDL is a typical biomarker for oxidative stress in HFD-induced obesity and related metabolic syndromes, we next used ox-LDL to treat primary peritoneal macrophages from WT and Pdcd4-/- mice, and examined TIA-1+ SGs in these macrophages. This evidence concerns the gene PDCD4 and obesity due to melanocortin 4 receptor deficiency.