Fully differentiated myotubes cultured from subjects with T2D displayed impairments in basal (11.57 ± 1.26 vs 18.48 ± 2.51 pmol/mg protein/min, T2D vs ND, p<0.05) and insulin-stimulated (15.52 ± 1.72 vs 20.76 ± 2.71, p = 0.10) glucose uptake, as well as b-oxidation of palmitate (14.05 ± 4.85 vs 35.36 ± 8.78 nmol/mg protein, p<0.05), similar to what we have reported previously [19, 21]. The gene discussed is INS; the disease is type 2 diabetes mellitus.