Here, we demonstrated that miR-192 negatively regulates myogenic differentiation through repressing the expression of RB1. Thus, we speculate that in LMNA-related muscular dystrophy, the up-regulation of miR-192 delays the expression of RB1 by directly targeting its 3′-UTR, and in turn, this may lead to abnormalities in skeletal muscle development. Here, RB1 is linked to muscular dystrophy.