In addition to those factors, we screened other possible breast cancer-derived factors that may be responsible for the enhanced directionality of mDC-MDA-MB-231 in a JNK signaling dependent manner, and found IL-1β and IL-6 cytokines, known to be related in enhancing the JNK signaling pathway48, 49, are highly expressed in MDA-MB-231 and MBA-MB-436 compared with those in MCF-10A or MCF-7. The gene discussed is IL1B; the disease is breast cancer.