TSC2 and lymphangioleiomyomatosis: Next, using two fibroblast cell lines that lack TSC2 (derived from TSC2 knockout mice and a lymphangioleiomyomatosis patient, respectively), we observed that increasing concentrations of SGK1-inh up to 30 μM were not able to reduce S6K signaling in these cellular models, as assessed by the downstream S6K targets pS6 (S235/6), pS6 (S240/4), and pmTOR (S2448) (Figure S5D).