Consistent with this, and with the strong downregulation in our data (to 71%; p = 7.6 × 10−10, GSE29079) as well as two validation prostate cancer tissue data sets (TCGA, Supplementary Figure S6A; Taylor et al., GSE21032), we hypothesized that the tumor-promoting role of miR-375 might be explained by targeting CBX7. To confirm the direct targeting of CBX7 by miR-375, we cloned the 3′-UTR of CBX7 into a luciferase reporter vector and measured the degree of signal reduction following miR-375 overexpression in PC-3 cells after 48 h. This evidence concerns the gene CBX7 and prostate cancer.