TFAP4 and neuroblastoma: We observed that knockdown of TFAP4 in both MYCN-amplified neuroblastoma BE(2)-C cells, and SH-EP/S1 neuroblastoma cells (stably overexpressing MYCN) resulted in a marked decrease in colony forming ability of these cells (Figure 2A), and cell cycle arrest at G1 and S phases (Figure 2B, Supplementary Figure S1C), while BE(2)-C cells also demonstrated a decrease in the cell population at G2/M phase.