To investigate the role of this transcription factor in inducing an EMT phenotype in MYCN-amplified neuroblastoma, we investigated a panel of eleven critical EMT-associated genes, including ACTN4, KRT8, MYH9, ROCK1, TCF3, VIM (Vimentin), ZEB1, TWIST (TWIST1), SNAIL (SNAI1), CDH1 (E-cadherin) and CDH2 (N-cadherin). The gene discussed is ACTN4; the disease is neuroblastoma.