PIM1 and melanoma: Using our 3D spheroid model of melanoma and western blot analyses, we observed that the combination of the PIM inhibitor SGI-1776 with the PI3Kβ inhibitor AZD6482 caused the lowest levels of pS6, pSTAT3, pAKT, p4EBP1, and increased levels of cleaved PARP (Figure 7B).