Since the subcutaneous model may not be fully informative on the glioma derived GL261 cells, we used an intracranial model to further explore the correlation of RAE-1 high expression and GL261 cell rejection in brains of the allogeneic mice, and obtained the results showing that the GL261 cell inoculated allogeneic BALB/c mice and ICR mice survived significantly longer than the control syngeneic C57BL/6 mice (Supplementary Figure S2B). Here, RAE1 is linked to central nervous system cancer.