In order to investigate whether the low or high expression of NKG2DL on the tumor cells could determine the tumor formation and rejection in allogeneic mice, we selected three strains of mouse-derived tumor cells, EMT-6 cells (BALB/c mouse origin), B16 cells (C57BL/6 mouse origin) and GL261 cells (C57BL/6 mouse origin) and tested expression levels of the NKG2DL including RAE-1, H60 and MULT-1 on the cells by flow cytometry. The gene discussed is RAE1; the disease is neoplasm.