IGFBP3 and neoplasm: Despite a positive association between serum MCP-1 and tumor volume (Supplementary Figure S4a) and a trend towards lower gene expression of Ccl2 in the mammary fat depot of BP3KO mice, tumor gene expression of the macrophage marker, Adgre1 (Supplementary Figure S4b), and Ccl2 (Supplementary Figure S4c), was similar among the four groups, suggesting that tumor infiltration by macrophages was not affected either by HFD feeding or by IGFBP-3.