In the meantime, to verify the underlying mechanism of the inhibitory effect of miR-7 on VemR melanoma tumor growth we also performed the immunohistochemistry (IHC) staining of EGFR, IGF-1R, CRAF, p-ERK and p-AKT and analyzed the total integrated optical density (IOD) of each parameter from both tumor groups. This evidence concerns the gene AKT1 and melanoma.