Similar results were observed after PXR gain-of function in two different LS174T clones (i.e. LS PXR2 and LS PXR6; Supplementary Figure S4a) in which microarray (Supplementary Table S3) and RT-qPCR validation analyses (Supplementary Figure S4b) confirmed that over-expression of PXR regulates the expression of multiple colon CSC markers (ALDH1A1, ABCG2, CYP3A4, CD24 [34], CXCR4 [35], LRIG-1 and OLFM4) and prognostic markers for colorectal tumor recurrence (ABCC6 [36] and S100A10 [37]). The gene discussed is CYP3A4; the disease is colorectal neoplasm.