After CRH injection, IBS patients showed significantly higher activity in the right amygdala compared to controls in a regions of interest (ROI) analysis (t = 3.63, cluster [k] = 42, ROI PFWE-corr = .017; local maximum—x: 34, y: 2, z: -22) (Fig 2). The gene discussed is CRH; the disease is irritable bowel syndrome.