LAG3 and melanoma: CD4+TIM3+ was increased from 12% (naïve lymph nodes) to 19% (draining lymph node), other IRs phenotype such as CD4+BTLA+ (38%), CD4+2B4+ (35%), CD4+LAG3+ (27%) was also increased in draining lymph node of melanoma-bearing mice than naïve mice (Figure 1A).