10 adult BCR-ABL1-positive B-ALL patients, 3 (marked as pI, pII, pIII) only expressed IK1(BCR-ABL1+-IK1), 3 (pIV, pV, pVI) only expressed IK6 (BCR-ABL1+-IK6), 2 only expressed IK2 (BCR-ABL1+-IK2) and 2 only expressed IK4 (BCR-ABL1+-IK4) were randomly selected, and their primary CD34+ leukemic cells (BCR-ABL1+-IK6 leukemic cells and IK6-negative leukemic cells, including BCR-ABL1+-IK1 leukemic cells, BCR-ABL1+-IK2 leukemic cells and BCR-ABL1+-IK4 leukemic cells) at diagnosis were used for transfection. The gene discussed is CD34; the disease is acute lymphoblastic leukemia.