As TRIM16 protein expression is known to induce apoptosis in neuroblastoma [35], we overexpressed TRIM16 in low TRIM16 expressing melanoma cell lines, Mel-JD, A753 and G361 and showed an increase in apoptosis in the TRIM16 overexpressing cells compared to empty vector controls (P < 0.05) (Supplementary Figure 4A). Here, TRIM16 is linked to neuroblastoma.