In addition, considerably elevated levels of ZKSCAN3 expression were observed in high-grade/muscle-invasive tumors, compared with low-grade/non-muscle-invasive tumors in the validation TMA, while there were no statistically significant associations between the status of ZKSCAN3 expression in these clinical samples we obtained at our institutions and tumor grade/stage. The gene discussed is ZKSCAN3; the disease is neoplasm.