We first examined the expression of ZKSCAN3 in human bladder cancer cell lines, UMUC3, 647V, and 5637, as well as an immortalized human normal urothelial cell line, SVHUC, by western blotting (Figure 1A) and reverse transcription (RT)-polymerase chain reaction (PCR) (Figure 1B). Here, ZKSCAN3 is linked to urinary bladder carcinoma.