In accordance with the data previously obtained upon stable silencing of HIF-1α (Figure 4D and 5B), acute HIF-1α silencing resulted in similar down-regulation of the top AML-M5 HIF-1α-target genes in the three cells lines, with the notable exception of LGALS1 and CDKN1A, which were consistently found not regulated or up-regulated respectively (Figure 6A, 6D and 6G). This evidence concerns the gene CDKN1A and acute myeloid leukemia.