ELF3 and carcinoma: Determination of specific time point and tissue context during development, instead of generic differentiation models using embryonic carcinoma cell lines (Hou et al., 2004; Park et al., 2014), will thus be more informative in designing in vitro models representative of the developmental stage of interest to study the role of ESE-1 during embryogenesis, and relate the information back to the in vivo setting.