The mRNA levels of NLRP3 inflammasome components, caspase-1 activity, and serum IL-1β were significantly increased in MCD and long-term HFD-induced mice model of NASH, but not in short-term HFD fed or leptin-deficient (ob/ob) mice [11]. This evidence concerns the gene NLRP3 and metabolic dysfunction-associated steatohepatitis.