In the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) group and polymerase chain reaction-polyacrylamide gel electrophoresis (PCR-PAGE) group, significantly decreased association between NFKB1 -94 ins/del ATTG promoter variant and cancer risk were identified, among which the most obvious is for recessive model in the two groups (PCR-RFLP group: DD vs. DW+WW: OR = 0.72, 95% CI = 0.60–0.87, P = 0.001; PCR-PAGE group: DD vs. DW+WW: OR = 0.65, 95% CI = 0.50–0.85, P = 0.002), respectively. This evidence concerns the gene NFKB1 and cancer.