Chloroplast expression system has helped address challenges in low expression level and a number of vaccines antigens against cholera, tetanus, anthrax, plague, polio or canine parvovirus achieving up to 13.17% and 10.11% of TSP in dual cholera and malaria vaccine expressing CTB‐fused apical membrane antigen 1 (AMA1) and merozoite surface protein 1 (MSP1) (Davoodi‐Semiromi et al., 2010) and >70% of TSP for CTB–proinsulin (Ruhlman et al., 2010). Here, INS is linked to vibrio infectious disease.