Contrary to these cells, SIK2 from the cancer cell lines (e.g., 293T cells) is readily solubilized with that buffer.39 To further dissect the sub-ER location of SIK2, we treated mesothelial cells with methyl-β-cyclodextrin (MβC), a reagent used to selectively extract cholesterol from the membrane. This evidence concerns the gene SIK2 and cancer.