The 6 candidate reference genes were: ACTB, HPRT, RPLPO, TBP, TFRC; beta-GUS And the expression was tested on 5 Healthy; 3 AA; 2 CRC and 3 IBS samples As can be seen in Fig B in S1 File, ACTB gene was expressed in early qPCR cycles (cycle 23–28), and beta-GUS gene was expressed in late qPCR cycles (34.5–39) and expression of TBP gene could not be detected in all samples. This evidence concerns the gene TFRC and irritable bowel syndrome.