GnRH agonist trigger have been shown to be beneficial in situations like repeated IVF failure, empty follicle syndrome and repeated retrieval of immature oocytes, with the hypothesis that some patients may require the FSH surge, in addition to the LH surge, to promote final oocyte maturation resembling the natural midcycle surge of gonadotropins (29,30). This evidence concerns the gene PLOD1 and Empty ovarian follicle.