Therefore, we introduced DLL4 or short hairpin shDLL4 into above HCC cells and conducted validation in vitro. The following loss- and gain-of-function assay showed that overexpression of DLL4 resembled SYNJ2BP-mediated proliferation and migration of HCC cells; conversely, silencing of DLL4 blocked SYNJ2BP function. This evidence concerns the gene SYNJ2BP and hepatocellular carcinoma.