Since ovarian cancers and breast cancers frequently express FRα, established human ovarian cancer cell lines (SKOV3) and breast cancer cell lines (T47D, SKBR3, and MCF7) that express surface FRα at varying levels were used for positive control targets, while FRα-negative cell lines C30 and AE17 were used as negative controls. The gene discussed is FOLR1; the disease is breast cancer.