Immunoblotting of tumor tissue confirmed that NVP-BEZ235 blocked PI3K (AKTSER473/308, GSK3βSER9) and mTOR signaling (RPS6SER240/244, 4EBP1THR37/46) coincident with down-regulation of intra-tumoral MYCN protein (Figure S4C). This evidence concerns the gene PIK3CA and neoplasm.