During the analysis for acute toxicity, we noticed that after injection of Tat-86, splenomegaly occurred in all the experimental mice, while the injection of Tat-R5M4 did not show any significant change (Figure 5c, d), suggesting that the immunogenicity of Tat-R5M4 was lower than that of Tat-86. This evidence concerns the gene TAT and Splenomegaly.