the number of cells detected relative to the number of cells spiked into the peripheral blood mononuclear cell (PBMC) fraction—we used 8 different cell lines: the human pancreatic cancer cell lines PANC1, BxPC3, and ASPC-1; the human breast cancer cell lines MCF7 (Luminal A), ZR-75-1 (Luminal B), MDA-MB-231 (Triple negative/basal), and MDA-MB-453 (HER2+); and the mesenchymal human mesothelioma cell line SDM103T2. The gene discussed is ERBB2; the disease is breast cancer.