Similarly, we have utilized the spheroid model to study the contribution of zonula occludens protein 1 (ZO-1) to the oncogenic behavior of melanoma [27], the response to MEK-inhibitors (MAPK pathway) and/or PI3K-inhibitors (PI3K/AKT/mTOR pathway) [23,24], BH3-mimetics (intrinsic apoptosis pathway) [20,21], and a novel class of anti-tropomyosin compounds (cytoskeleton) [28]. This evidence concerns the gene MAP2K7 and melanoma.