KRAS and intrahepatic cholangiocarcinoma: In a retrospective study of case series of 153 surgically-resected primary biliary cancers (including 70 ICC, 57 ECC and 26 GBC) derived from patients of Italian origin, the multigene next generation sequencing (NGS) analysis demonstrated that 43/153 (28.1%) of patients presented K-RAS mutation; in particular, subdividing for histological groups, 47.4% of ECC shared K-RAS mutations, while ICC and GBC 15.7% and 19.2%, respectively [25].