Notably, the pooled SEN was much higher in high-incidence areas than in low-incidence areas, but the SPE was lower, indicating that in high-incidence areas of HCC, IHC assays for p53 expression accurately predicted p53 alterations with authentic genetic mutations but only showed modest accuracy in identifying wild-type p53 phenotypes with no p53 protein overexpression. The gene discussed is TP53; the disease is hepatocellular carcinoma.