Of note, there was no statistical significance between any two of the experimental groups, suggesting that the increased maturation of autophagosomes into autolysosomes after AP induction was immune to the further interventions against the incubated level of H2S. In vivo, an immunocolocalization assay between LAMP‐2 and LC3 was conducted. The gene discussed is MAP1LC3A; the disease is alkaline phosphatase measurement.