We explored PBMC-based stage-dependent gene signatures using stage-stratified subpopulations and specifically identified two advanced stage-dependent genes (STAT2 and WEE1), in addition to six common NSCLC-associated genes (EIF2S3, DUSP6, GRB2, MDM2, POLDIP2, and RNF4). This evidence concerns the gene RNF4 and non-small cell lung carcinoma.