We identified 11 quantitative PCR-based NSCLC-associated markers using logistic regression analyses in our age- and gender-matched case–control study; these included CPEB4, DUSP6, EIF2S3, GRB2, MCM4, MDM2, NF1, POLDIP2, RNF4, STAT2, and WEE1. These statistically significant markers in our study were very different from those of other blood-based studies. This evidence concerns the gene RNF4 and non-small cell lung carcinoma.