PTPRC and cancer: Using cancer cell lines spiked in normal blood and filtered by ISET, we developed a multi-step process where filters were (i) treated by four-color fluorescent staining, (ii) scanned using an Ariol scanning system and automatically analyzed to select CD45− from CD45+ cells, (iii) treated by a cytomorphological dye, (iv) scanned and analyzed again using the Ariol scanning system to gather for cells of interest phenotypical and cytomorphological images, and cell characteristics (Fig. 1a).