F‐actin staining (Fig. S3A,B) and RT–PCR analysis (Fig. S3C) revealed that 8,9‐EET, 11,12‐EET, and 14,15‐EET prevented PE‐induced cardiac hypertrophy, but that 11,12‐EET exerted the greatest effect and increased ANP expression more compared with PE stimulation (Fig. S3D,E). This evidence concerns the gene NPPA and cardiac hypertrophy.