KRAS and colorectal cancer: Interestingly, no differences in the response to STLC, measured by a short-term apoptosis assay (Supplementary Fig. S9e) or by a long-term colony forming assay (Supplementary Fig. S9f), were observed when comparing isogenic colorectal cancer cell lines HCT116 KRAS+/− and KRAS+/G13D, which were previously used to uncover sensitivity to several mitotic perturbations such as inhibition of PLK12.