Simulations (n = 24) revealed that applying the TGFβ inhibitor in silico at day 1 prior to the seeding of the cancer cells (pre-treatment scenario) even at a level of 20% efficacy significantly reduced tumor burden over time by ≥65% (Fig 1b, Supplementary Fig. S2, Table S3, and Video 2). The gene discussed is TGFB1; the disease is cancer.