In addition, we have also measured the effect of CHST15 siRNA on the expression of EMT markers such as E-cadherin, vimentin, BMP7 and Wnt3 using TGF-β-stimulated colon cancer cell line and identified a significant suppression of these markers in the CHST15 siRNA treated cells (Fig 6E). This evidence concerns the gene TGFB1 and malignant colon neoplasm.