Analogous to murine parental HER-2/neu+ cells, hypoxia increased pCREBSer133 expression in the breast cancer cell line HTB122 transfected with functional HER-2/neu (E2), but not in HTB122 cells transformed with a signaling-deficient HER-2/neu construct (E2A, Figure 2E). This evidence concerns the gene ERBB2 and breast cancer.