To further elucidate the influence of the AIM2 inflammasome on M. bovis-induced autophagy, immunofluorescence was used to detect the amounts of LC3 and SQSTM1/p62 in BMDMs at 24 h post-infection and we saw increased LC3 and decreased SQSTM1/p62 (Figure 3d and 3e). Here, SQSTM1 is linked to infection.