To verify this hypothesis, we carried out the present work and found the activation of autophagy during infection of M. bovis did not require the AIM2 inflammasome, on the contrary, it is interesting to note that the siRNA-mediated disruption of AIM2 significantly increased autophagy and also found an increase of the co-localization of selective autophagy marker p62 and LC3 with M.bovis. This evidence concerns the gene SQSTM1 and infection.