Murine bone marrow monocyte-derived macrophages (BMDMs) were infected with M. bovis at a multiplicity of infection (MOI) of 10, and the level of the autophagy marker, microtubules associated protein light chain 3 (LC3) in cell lysate detected by immunoblotting at 0h, 4 h, 12 h, and 24 h post-infection. The gene discussed is MAP1LC3A; the disease is infection.