The α7 subunits formed functional nAChRs in the NSCLC cells, because the cells responded to nicotine by an increase of intracellular calcium influx (Figure 1B, 1C, 1D, 1E, 1F, 1G, and 1H) and the effect was hampered by the α7-nAChR selective antagonist α-bungarotoxin (α-BTX) (Figure 1B, 1C, 1F, and 1G) or by the knockdown of the α7 subunit via RNA interference (Figure 1D, 1E, 1H, and 1I). The gene discussed is CHRNA7; the disease is non-small cell lung carcinoma.