To further investigated whether HDACIs can affect the mRNA stability of ABCB1 in colorectal cancer cells, HCT116 and SW480 cells were treated with DMSO, SAHA or TSA for 24 h, after which the transcription inhibitor actinomycin D (Act-D) was added for the following 24 h to inhibit nascent RNA synthesis. Here, ABCB1 is linked to colorectal cancer.