The above data demonstrated that the interaction between 14-3-3ζ and aPKC-ι promote the activated form of them by phosphorylation, and activation of aPKC-ι lead to activate the GSK-3β/Snail signaling pathway by p-GSK-3β, and promotes the EMT ultimately in CCA cells (Figure 7D). Here, GSK3B is linked to cholangiocarcinoma.