TP53 and non-small cell lung carcinoma: In this study, we evaluate the performance of a recently developed single allelic molecule counting methodology termed single molecule amplification and re-sequencing technology (SMART) [34] for the purpose of detecting and quantitating hot spot EGFR, KRAS, BRAF, ALK and TP53 mutations in NSCLC tumour specimens and define mutation profiles of early and advanced stage disease.