Consistent with the staining, significant increases in the levels of tau phosphorylated at Thr231, Thr205, Ser396, and Ser404 were evident in the hyperglycemia-exposed animals compared with the APP/PS1 mice; there were similar changes in the brains of the Pdx1+/− mice compared with the WT mice (p < 0.01 or p < 0.05, respectively; Fig. 5B–F). Here, PDX1 is linked to Hyperglycemia.