In conclusion, a total of 393 DEGs were identified between osteocytes co-cultured with and without myeloma cells, and KLF4, IRF8, EGF, EGR1, S1PR1, C3AR1, and NPY1R might be involved in osteocyte cell apoptosis induced by MM cells. This evidence concerns the gene KLF4 and Miyoshi myopathy.