In the study of 90 patients with ccRCC and 20 normal renal tissue samples, hyper-methylation of IGFBP-3 was not found, but in 786–0, ACHN, HRC51 and HRC59 cell lines methylation of IGFBP-3 was observed [81]. The gene discussed is IGFBP3; the disease is nonpapillary renal cell carcinoma.