In conclusion, the present study has demonstrated that TXYF was able to regulate 5-HT-induced Cl− and HCO3− secretion in D-IBS-like rats, possibly mediated by the transport elements as CFTR, NKCC, Na+-HCO3− cotransporter, Cl−/HCO3− exchanger, K+ channel, and Na+/K+-ATPase, at least in part. This evidence concerns the gene CFTR and irritable bowel syndrome.